Phytochemical profile, antimicrobial potential and GC-MS analysis of wild variety of Olea Europaea (Olive) cultivated in Pakistan
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Abstract
Olive plant produces a variety of bioactive molecules and thus has important medicinal value in folk medicine. In this study, different leaf and fruit extracts of Pakistani wild variety of Olea europaea was tested for their phytochemical content, antimicrobial activity and mass spectrometric analysis. Olive leaves and fruit samples were extracted with five different solvents to obtain the crude extract and screened for various kinds of phytochemicals. Phytochemicals were further confirmed through Fourier Transmission Infra-red Spectroscopy (FTIR).The plant extract showed significant antimicrobial activity against all the strains tested. Methanol, ethanol and ethyl acetate extracts were found more effective against most of the pathogenic bacteria with high zone of inhibition. Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed that olive fruits have Oleic acid, Palmitic acid, Linoleic acid, Octadecadienoic acid, Stearic acid, Palmitoleic acid and Tridecanoic acid as oil contents. In this work, the antimicrobial potential and phytochemical contents were explored which may further pave the way for the bio-industrial applications.
Keywords: Olea europaea; Phytochemical content; Antimicrobial activity; GC-MS analysis