1. Analysis of FecG gene in lohi sheep breed using PCR-RFLP technique

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Published Mar 2, 2018

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Hamid Mustafa
Department of Livestock Production, University of Veterinary and Animal Sciences, Ravi Campus, Pattoki-Pakistan
Waqas Ahmad Khan
Department of Biotechnology, Faculty of Science, University of Sargodha, Sargodha-Pakistan
Zulfiqar Hussain Kuthu
Faculty of Veterinary and Animal Sciences, University of Poonch Rawalakot, Azad Kashmir-Pakistan
Adeela Ajmal, Khalid Javed
Department of Livestock Production, University of Veterinary and Animal Sciences, Ravi Campus, Pattoki-Pakistan
Noor Ul Ain
The University of Agriculture, Peshawar-Pakistan
Ahmad Ali
Department of Livestock Production, University of Veterinary and Animal Sciences, Ravi Campus, Pattoki-Pakistan
Muhammad Zeeshan
Department of Animal Sciences, University of Sargodha-Pakistan

Abstract

The growth and follicles differentiation in ovary is controlled by the FecG gene of TGF-β superfamily proteins. FecG (GDF9), FecB (BMPRIB) and FecX (BMP15) are three oocyte-derived genes of TGF- β superfamily, which have essential role in ovulation and follicular growth. This study was intended to identify the FecG mutation in 30 ewes of Lohi sheep. A fragment length of 139 bp of FecG gene at exon 2 was amplified with a forced PCR-RFLP primer pairs and products were digested with Ddel enzyme. The results revealed that there is no mutation at this locus, because Ddel enzyme could not recognize restriction site. In this study, we found wild type (++) alleles. The polymorphism analysis of Lohi sheep indicates that the reported mutation for litter size is not present in this breed. Therefore, it is important to identify other SNPs for FecG gene in Lohi sheep.


Keywords: Allele; FecG; Lohi sheep; Mutation; Polymorphism


http://dx.doi.org/10.19045/bspab.2018.70035

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How to Cite
MUSTAFA, Hamid et al. 1. Analysis of FecG gene in lohi sheep breed using PCR-RFLP technique. Pure and Applied Biology (PAB), [S.l.], v. 7, n. 1, p. 292-295, mar. 2018. ISSN 2304-2478. Available at: <https://mail.thepab.org/index.php/journal/article/view/390>. Date accessed: 18 mar. 2025.
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Issue
Vol 7 No 1: March-2018
Section
Short communication

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