18. Antimicrobial evaluation of various leaves extracted samples of nettle desert (Forsskaolea tenacissima L.)
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Abstract
The present study evaluates the anti-microbial activity of three different crude extracts (ethanol, aqueous and n-hexane) of Forsskaolea tenacissima L. leaves against gram negative and gram positive bacteria and fungi using well diffusion method. N-hexane extract showed tremendous inhibition of 12mm (80% ZI) and 10mm (71.42% ZI) against Staphylococcus aureus and Bacillus subtillis at 1000 µg/ml concentration. Similarly, aqueous extract at the concentration of 1000 µg/ml reduced the growth of Xanthomonas maltophilia and Escherichia coli as 11mm (68.75% ZI) and 9mm (60.00%), respectively. However, ethanol extract showed good activity of 12mm (75.00%) against Clavibacter michiganense Aqueous extract showed 9mm (75.00%) against Acromonium alternatum at 1000 µg/ml. Rhizopus stolinifer and Trichoderma reesei both were found sensitive to aqueous extract, which showed 11 and 8mm (68.75 and 57.14% ZI) at 1000 µg/ml concentration, respectively. The growth of Aspergillus niger was inhibited by ethanol extract through 9mm (56.25% ZI) at the concentration of 1000 µg/ml. The above study determined the medicinal importance of Forsskaolea tenacissima.
Keywords: Antimicrobial activity; Bacteria; Forsskaolea tenacissima; Well diffusion method