06. Isolation and identification of heavy metal tolerant bacteria from volta battery effluent, Hattar, Haripur Pakistan
Abstract
Use of microorganisms to remediate contaminated water is cheap, rapid, easy and eco friendly methodology. In present investigation bacterial isolates from Volta Battery effluent Hattar, Haripur are studied for tolerance to Copper, Cobalt, Lead and Zinc salts. Four isolates i.e. Staphylococcus aureus, Pseudomonas aeruginosa, Citrobacterfreundii and Rahnella selected after initial screening were further assayed by time kill assay. Growth kinetics of bacterial cultures and metal accumulation capability are also examined.Pseudomonas aeruginosawas found resistant to tested metals at varying concentrations however Zinc badly influenced bacterial growth. Highest metals uptake efficiency by strains was noted for Cobalt followed by Zinc; 87 and 81 %, respectively. Staphylococcus aureus accumulated metals at greater extent as compared to other strains tested. The results suggests that species isolated from Volta Battery effluent can further be investigated for mechanisms that make them able to grow in high metal concentrations and can be applied to contaminated sites for remediation.
Keywords: AAS; Effluent; Heavy metals; Hattar industrial state; Optical density
