22. Biochemical and morpho-genetic characterization of wild and cultivated Pomegranate (Punica granatum L.)
Main Article Content
Abstract
Present research was conducted to characterized the cultivated and wild pomegranate on the basis of morphological and biochemical trait. For morphological characterization fruit weight, fruit length, fruit width, peel thickness, number of arils and leaf area were evaluated. The studied parameters fruit weight, fruit length, fruit width, peel thickness, number of arils and leaf area were within the range of 36-215(g), 30-66.9(mm), 8.45-64.61(mm), 0.2-4.2(mm),180-490 and 1.4-9.5(cm2) respectively. Cluster analysis (UPGMA) and Principal Component Analysis (PCA) for morphological trait reflected significant difference between cultivated and wild pomegranate. Biochemical characterization of cultivated and wild pomegranate was investigated through protein banding profile using Sodium Dodecyl Polyacrylamide Gel Electrophoresis (SDS-PAGE). For this purpose, the protein marker of 10-220 kDa (Novex by life technologies) were used to assess the protein polymorphisms within wild and cultivated species of pomegranate and clarify the genetic nature of polymorphic bands. On the basis of banding pattern, electrophorogram were sketched by which molecular weight of species specific band were calculated. The results showed that both wild and cultivated pomegranate conferred genetic diversity and depicted low level of heterogeneity.
Keywords: Morphological traits; Protein marker; Wild and cultivated pomegranate; Cluster analysis