Expressional studies of microRNAs in Hepatitis B Patients of Quetta, Pakistan
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Abstract
Hepatitis B (HB) is a severe and widespread infectious liver disease affecting millions of people throughout the globe and caused by hepatitis B virus (HBV). Persistent HBV infections results severe pathologies which include development of chronic hepatic insufficiency, cirrhosis, and hepatocellular carcinoma (HCC). In response of the HBV, patient cells show differential expression of microRNAs (miRNAs) to manage and fight the HB. miRNAs are small, endogenous and regulatory RNAs, that play an important role in post-transcriptional gene regulation. The aim of this research is to study the expressional pattern of fifteen miRNAs; hsa mir-20, 21, 30, 99, 122, 141, 190, 200, 223, 320, 574, 1228, 4532, 6127 and 6852 in hepatitis B patients of Quetta, Pakistan, selected through literature survey on the basis of their significant role in hepatitis. Briefly, the total RNA from the hepatitis B patient of Quetta, Pakistan and healthy person extracted and subjected to the stem-loop RT-PCR (reverse transcriptional-PCR) for the selected fifteen miRNAs and GAPDH-as internal control gene. The eight miRNAs; has-mir-20, 21, 30, 99, 141, 574, 4532 and 6852 showed upregulation, whereas, the six miRNAs; has-mir-122, 190, 200, 223, 320, and 6127 showed down-regulation in HB-patient. One miRNA; 1228 is observed with equal expression in both patient and healthy person. Hepatitis responding miRNAs would be useful to better understand the disease for early diagnose and cure.
Keywords: HBV; microRNAs; RT-PCR