08. Beta-D-fructofuranosidase production by Aspergillus niger IBGE 01 using shaken flask technique of submerged fermentation
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Abstract
The modern biotechnological set up has motivated the need of enlarged survey of microorganisms which could be utilised in the extreme conditions of industries. In this work optimization parameters in submerged fermentation were studied for the production of Invertase from Aspergillus niger IBGE 01 using agricultural wastes (sunflower waste, cotton stalk and rice husk) as well as agro industrial wastes(date syrup and molasses) as sources of carbon. Effects of incubation time period (24-240 h), various cultural media (CM1, CM2, CM3, CM4 and CM5) sources of nitrogen (Corn steep, Casein, Potassium Nitrate Albumin Ammonium Sulphate Urea and Yeast Extract), pH (4.0-9.0), temperatures (30-70° C), inoculum size (4x106-8x106 conidia/mL) and agitation rate (50-300 rev/min) were also investigated for maximum invertase production. Optimal conditions for the production of invertase (8.23 U/mL) by Aspergillus niger IBGE 01 were observed when the strain was grown on culture medium CM1 containing yeast extract as a source of nitrogen, molasses as a source of carbon after 72 h of incubation at 40° C, initial pH 6.0, inoculum size of 5x106 conidia in 50 mL of culture medium and agitation rate of 150 rev/min. The strain was proved pH (up to 9) and thermo stable (up to 60° C) therefore can be used in industries for invertase production.
Keywords: Invertase; Aspergillus niger; Optimization