15. PCR based DNA fingerprinting of mercury resistant and nitrogen fixing Pseudomonas spp.
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Abstract
Mercury resistant Pseudomonas strains were isolated from polluted water, rhizospheric soil and root nodules of different plant species. Selected bacterial isolates showed potential to produce florescence under UV light and to tolerate heavy metal stress of 10-20 μg/ml HgCl2. Biochemical characterization revealed all selected bacterial isolates belong to genus Pseudomonas. A total of 27 bacterial isolates were evaluated for bioremediation of mercury due to their ability to produce H2S and fix nitrogen. Genomic DNA of 12 Pseudomonas strains was isolated and their genetic diversity was evaluated using SSR (GACA)4 microsatellite finger printing. On the basis of banding pattern of PCR amplified products, a phylogenetic tree was constructed using Minitab 13 software which showed 71%, 46% and 44% homology of SZ-30, SZ-6 and SZ-8 with strains of cluster 1 and SZ-16 respectively. Results exhibited significant diversity among selected Pseudomonas strains.
Keywords: Microsatellite-primed PCR; Fluorescent Pseudomonads; Hg-resistance