01. Studies on genetic diversity of cotton using RAPD markers
Abstract
The study was conducted to assess the genetic diversity in 12 cotton varieties (Gossypium hirsutum). Diverse gene
pool is significantly important in variety development programs and assessment of genetic variability is the first step
in any crop improvement programs. The results showed considerable variability in the genotypes under study. Four
RAPD primer series (A, C, J & K) were used in this study. Most of the primers used showed significant
polymorphism. The genotypes were clustered into four groups (A, B, C and D). Four groups contained cluster and
sub clusters which showed the similarity and dissimilarity of the genotypes. In group A, there were four genotypes,
FH-169, FH-170, FH-172 and FH-113 while in group B, FH-941, CIM-241, CRIS-134 and PB-900 were present. In
group C and D, there were three varieties i.e. CIM-XCP 29, CIM-506, CIM-448 and one genotype (CIM-240)
respectively. This information would be useful for cotton breeding programs and in the hybridization experiments.
Keywords: Cotton, Genetic Diversity, PCR, RAPD Markers
