02. Parental bias in segregation distortion of RAPD markers from Solanum anther-derived plants
Abstract
A diploid Solanum clone 9507-04 was used to grow anther-derived plantlets that were putatively monoploid. Sixteen RAPD primers were used to observe the RAPD profiles from anther-donor and anther-derived materials. A total of 56 RAPD markers were scored and each marker was identified according to the primer’s designation and size (kilo base pairs) of the amplified fragment. Of 56 RAPD markers assayed, 44.6% did not follow the expected 1:1 Mendelian segregation (χ2>4.26, P<0.05) in the anther-derived progeny. The selected six morphological traits also segregated in anther-derived plants including the nine that retained 3-4 of the parental leaf characters. The 12 distortion–causing RAPD markers (χ2=8.89-17.64, P<0.003) with above 84% presence in the progeny were retained in all above nine plants. Such preference of parental markers in potato anther-derived progeny may indicate a linkage with androgenesis.
Keywords: Anther culture, parental-type, polymorphism, potato
