04. Molecular diagnostics of foodborne pathogens
Abstract
All over the world Shiga toxin producing E. coli (STEC) are frequently cause of GIT illnesses in human originating in food of animals mainly from poultry.The aim of this study was to render rapid diagnostics of Shiga toxin producing E. coli (STEC) from raw meat and milk. During a 4 month period from May to August a total of 300 samples were collected from beef (100), chicken (100) and milk (100) and analyzed for Shiga toxin producing E. coli. Of the total of 300 samples of raw meat and milk only chicken (2%), beef (1%) and milk (1%) were detected as PCR positive.Raw meat and milk could be a source of Shiga toxin producing E. coli which indicates that possible risks of infections to people could be transferred by the consumption of raw meat and milk and their rapid diagnostics could be made possible by the use of rapid diagnostic technique polymerase chain reaction.
