Attributes of bioactive compounds isolated from commercial brands of fenugreek (Trigonella foneum-graecum) in relation to organic solvent systems and their potential as antioxidants and biological activity

Abstract

The present study is about potential variations of bioactive compounds from different commercial brands of fenugreek; in relation to multiple organic solvent systems, antioxidants and antimicrobial assays. Total phenolic contents, total flavonoid contents, individual phenolic acids, and DPPH assays were checked whereas phenolics analysis was performed by HPLC spectrophotometrically. Antimicrobial activity of different extracts was ascertained by disc diffusion method. The crude extract yields for different commercial brands of fenugreek ranged from 35.53-62.77 g/100g dry weight. Maximum yield was obtained from 100% Local Fenugreek with methanol while minimum was obtained from 100% local fenugreek chloroform. A considerable amount of TPC and TFC 24.4- 42.60 mg of Gallic acid equivalents per g of sample dry weight (mg GAE/g DW) and 0.75-1.87 as mg of catechin equivalents per gram of sample dry weight (mg CE/g DW) respectively was observed. The amount of DPPH and reducing power essay was 0.927-3.78 mg/mL and 4.20-7.76 mg/mL respectively. Different brands of fenugreek had significant amount of phenolics acids ranging from 14.36-1035.44 mg/kg, where Gallic acid was found in all extracts. Antimicrobial activity was tested against microorganisms and were found to be considerable significant. These finding indicates that fenugreek has strong potential to be used as pharmaceuticals, antioxidants and preservatives.

Keywords: Antioxidant activity; Biological activity; Extraction efficacy of solvents; Total phenolics; Total flavonoids

http://dx.doi.org/10.19045/bspab.2017.60092